Gene Rv3720
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involved in lipid metabolism. |
| Product | Possible fatty acid synthase |
| Comments | Rv3720, (MTV025.068), len: 420 aa. Possible fatty-acyl-phospholipid synthase, equivalent to Q9CB74|ML2334 (alias O69515|MLCB2407.16c, 439 aa) hypothetical protein from Mycobacterium leprae (420 aa) FASTA scores: opt: 2508, E(): 4.7e-153, (86.45% identity in 420 aa overlap). Also similar (especially at the C-terminus) to various fatty-acid synthases (principally cyclopropane-fatty-acyl-phospholipid synthases) and hypothetical proteins e.g. Q9KZ58|SCE25.32c putative fatty acid synthase from Streptomyces coelicolor (438 aa), FASTA scores: opt: 1101, E(): 5.5e-63, (46.1% identity in 425 aa overlap); P31049|YLP3_PSEPU hypothetical 44.7 KDA protein from Pseudomonas putida (394 aa), FASTA scores: opt: 810, E(): 2.1e-44, (46.4% identity in 293 aa overlap); Q9HT28|PA5546 hypothetical protein from Pseudomonas aeruginosa (394 aa), FASTA scores: opt: 804, E(): 5.2e-44, (40.7% identity in 371 aa overlap); Q9RSD7|DR2187 putative cyclopropane-fatty-acyl-phospholipid synthase from Deinococcus radiodurans (462 aa), FASTA scores: opt: 747, E(): 2.6e-40, (35.95% identity in 409 aa overlap); BAB50831|Q98ET6|MLL4091 cyclopropane-fatty-acyl-phospholipid synthase from Rhizobium loti (Mesorhizobium loti) (422 aa), FASTA scores: opt: 674, E(): 1.1e-35, (39.1% identity in 284 aa overlap); P30010|CFA_ECOLI|CDFA|B1661 cyclopropane-fatty-acyl-phospholip synthase from Escherichia coli strain K12 (381 aa), FASTA scores: opt: 530, E(): 1.7e-26, (33.65% identity in 312 aa overlap); etc. Also similar to other proteins from Mycobacterium tuberculosis e.g. CMA2|Rv0503c|MTCY20G9.30c (302 aa); P96911|Rv0621|MTCY20H10 (354 aa); O50416|LPQD|Rv3390|MTV004.48 (236 aa); etc. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4163736 | 4164998 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3720|Rv3720
MAEILEIFTATGQHPLKFTAYDGSTAGQDDATLGLDLRTPRGATYLATAPGELGLARAYVSGDLQAHGVHPGDPYELLKTLTERVDFKRPSARVLANVVRSIGVEHILPIAPPPQEARPRWRRMANGLLHSKTRDAEAIHHHYDVSNNFYEWVLGPSMTYTCAVFPNAEASLEQAQENKYRLIFEKLRLEPGDRLLDVGCGWGGMVRYAARRGVRVIGATLSAEQAKWGQKAVEDEGLSDLAQVRHSDYRDVAETGFDAVSSIGLTEHIGVKNYPFYFGFLKSKLRTGGLLLNHCITRHDNRSTSFAGGFTDRYVFPDGELTGSGRITTEIQQVGLEVLHEENFRHHYAMTLRDWCGNLVEHWDDAVAEVGLPTAKVWGLYMAASRVAFERNNLQLHHVLATKVDPRGDDSLPLRPWWQP
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
