Gene Rv3721c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [catalytic activity: N deoxynucleoside triphosphate = N pyrophosphate + DNA(N)]. |
| Product | DNA polymerase III (subunit gamma/tau) DnaZ/X |
| Comments | Rv3721c, (MTV025.069c), len: 578 aa. Probable dnaZX, DNA polymerase III gamma (dnaZ) and tau (dnaX), equivalent to O69514|DNAZX|ML2335 DNA polymerase III subunit gamma/tau from Mycobacterium leprae (611 aa) FASTA scores: opt: 2344, E(): 4.7e-118, (78.75% identity in 602 aa overlap). Also highly similar to many e.g. Q9RKL5|DNAZ from Streptomyces coelicolor (784 aa) FASTA scores: opt: 1755, E(): 1.8e-86, (59.55% identity in 435 aa overlap); Q9KGM4|DNAX|BH0034 from Bacillus halodurans (564 aa), FASTA scores: opt: 946, E(): 2.5e-43, (37.4% identity in 460 aa overlap); P09122|DP3X_BACSU|DNAX|DNAH from Bacillus subtilis (563 aa), FASTA scores: opt: 841, E(): 1e-37, (30.8% identity in 510 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). |
| Functional category | Information pathways |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4164995 | 4166731 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3721c|dnaZX
VALYRKYRPASFAEVVGQEHVTAPLSVALDAGRINHAYLFSGPRGCGKTSSARILARSLNCAQGPTANPCGVCESCVSLAPNAPGSIDVVELDAASHGGVDDTRELRDRAFYAPVQSRYRVFIVDEAHMVTTAGFNALLKIVEEPPEHLIFIFATTEPEKVLPTIRSRTHHYPFRLLPPRTMRALLARICEQEGVVVDDAVYPLVIRAGGGSPRDTLSVLDQLLAGAADTHVTYTRALGLLGVTDVALIDDAVDALAACDAAALFGAIESVIDGGHDPRRFATDLLERFRDLIVLQSVPDAASRGVVDAPEDALDRMREQAARIGRATLTRYAEVVQAGLGEMRGATAPRLLLEVVCARLLLPSASDAESALLQRVERIETRLDMSIPAPQAVPRPSAAAAEPKHQPAREPRPVLAPTPASSEPTVAAVRSMWPTVRDKVRLRSRTTEVMLAGATVRALEDNTLVLTHESAPLARRLSEQRNADVLAEALKDALGVNWRVRCETGEPAAAASPVGGGANVATAKAVNPAPTANSTQRDEEEHMLAEAGRGDPSPRRDPEEVALELLQNELGARRIDNA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
