Gene Rv3725
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Possible oxidoreductase |
| Comments | Rv3725, (MTV025.073), len: 309 aa. Possible reductase, similar to various oxidoreductases and hypothetical proteins e.g. O34285|HPNA HPNA protein from Zymomonas mobilis (337 aa), FASTA scores: opt: 317, E(): 6.1e-11, (30.5% identity in 272 aa overlap); Q9SZB3|F17M5.120|AT4G33360|AAK49584 hypothetical 37.9 KDA protein from Arabidopsis thaliana (Mouse-ear cress) (344 aa), FASTA scores: opt: 314, E(): 9.1e-11, (30.35% identity in 267 aa overlap); AAK59445|AT4G33360 putative dihydrokaempferol 4-reductase from Arabidopsis thaliana (Mouse-ear cress) (332 aa), FASTA scores: opt: 313, E(): 1e-10, (30.8% identity in 263 aa overlap); Q9FSC6|CCR cinnamoyl-CoA reductase from Populus trichocarpa (Western balsam poplar) (338 aa), FASTA scores: opt: 305, E(): 2.9e-10, (30.3% identity in 274 aa overlap); Q9M631 cinnamoyl CoA reductase from Populus tremuloides (Quaking aspen) (337 aa), FASTA scores: opt: 291, E(): 1.8e-09, (30.15% identity in 272 aa overlap); P73212|DFRA_SYNY3|LR1706 putative dihydroflavonol-4-reductase (dihydrokaempferol 4-reductase) from Synechocystis sp. strain PCC 6803 (343 aa), FASTA scores: opt: 278, E(): 1e-08, (29.35% identity in 259 aa overlap); etc. Also some similarity to proteins from Mycobacterium tuberculosis e.g. P96816|Rv0139|MTCI5.13 hypothetical protein (340 aa) FASTA scores: opt: 234, E(): 3.2e-06, (28.25% identity in 269 aa overlap); and O06373|galE1|Rv3634c|MTCY15C10.18 probable UDP-glucose 4-epimerase (314 aa) (27.3% identity in 194 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4170214 | 4171143 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3725|Rv3725
MQNATMRVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPARLKTSVAKLGVDVSDFAVADISDRDSVREALNGCDAVVHSAALVATDPRETSRMLSTNMAGAQNVLGQAVELGMDPIVHVSSFTALFRPNLATLSADLPVAGGTDGYGQSKAQIEIYARGLQDAGAPVNITYPGMVLGPPVGDQFGEAGEGVRSALWMHVIPGRGAAWLIVDVRDVAALHAALLESGRGPRRYTAGGHRIPVPELAKILGGSPAPRCWPSRCPIPRCVSRDRCWIKPGPICLSILRSPRQVCSTTHRCRSPTIRRAKKN
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
