Gene Rv3736
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Transcriptional regulatory protein (probably AraC/XylS-family) |
| Comments | Rv3736, (MTV025.084), len: 353 aa. Probable transcriptional regulator, araC/xylS family, similar to many transcriptional regulators and hypothetical proteins e.g. CAC38740 hypothetical 35.4 KDA protein from Bradyrhizobium japonicum (318 aa), FASTA scores: opt: 438, E(): 2e-20, (29.4% identity in 306 aa overlap); Q9HZ25|PA3215 probable transcriptional regulator from Pseudomonas aeruginosa (337 aa), FASTA scores: opt: 395, E(): 1.1e-17, (30.3% identity in 320 aa overlap); Q9HTN1|PA5324 probable transcriptional regulator from Pseudomonas aeruginosa (356 aa), FASTA scores: opt: 313, E(): 1.8e-12, (25.85% identity in 329 aa overlap); Q9Z3Y6|PHBR transcriptional regulator PHBR from Pseudomonas sp. 61-3 (379 aa), FASTA scores: opt: 271, E(): 8.3e-10, (22.95% identity in 357 aa overlap); etc. Also highly similar to Q06861|VIRS_MYCTU|Rv3082c|MTV013.03c possible virulence-regulating protein from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 656, E(): 3.7e-34, (36.95% identity in 333 aa overlap); and similar to other hypothetical mycobacterial proteins e.g. P71663|YD95_MYCTU|Rv1395|MT1440|MTCY21B4.12 (344 aa). Contains helix-turn-helix motif at aa 245-266 (Score 1140, +3.07 SD). Seems belong to the AraC/XylS family of transcriptional regulators. |
| Functional category | Regulatory proteins |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4186634 | 4187695 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3736|Rv3736
MSVVRGTALANYPSLVAGLGGDPATLLRAAGVRDQDVGNYDAFISIRAAIRAIESAAAVTATMDFGRRLAQRQGIEILGPVGVAARTAATVGDALAIFNTFMAAYSPVIAIRITPLAGQRSFIALEFLLDEPASYPQTMELALGVALGVIRLLLGADYAPLAVHLPHDPLTPEAFYLQYFGCRPYFAERVGGFTMRTADLSRPLNRDDVAHRVVVDYLSSITPLGEGIVESVRTIVRQLLPTGAATLNVVAEQFHLHPKTLQRRLAEENTTFVILVDRVRKDVADRYLRTTGIGLTHLARELGYAEQSVLTRSCKRWFGTGPAAYRNQARLQTTVSAPGSGRGPNPGNVSVSC
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
