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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in tyrosine biosynthesis [catalytic activity: prephenate + NAD(+) = 4-hydroxyphenylpyruvate + CO(2) + NADH].
ProductPrephenate dehydrogenase TyrA (PDH) (hydroxyphenylpyruvate synthase)
CommentsRv3754, (MTV025.102), len: 301 aa. Probable tyrA, prephenate dehydrogenase, equivalent, but shorter 27 aa, to Q9CB34|ML2472 possible prephenate dehydrogenase from Mycobacterium leprae (327 aa) FASTA scores: opt: 1600, E(): 1.6e-89, (80.0% identity in 300 aa overlap). Also similar to many pephenate dehydrogenases e.g. Q9RND8|TYRA from Bordetella bronchiseptica (Alcaligenes bronchisepticus) (299 aa), FASTA scores: opt: 345, E(): 9.7e-14, (32.85% identity in 271 aa overlap); Q9RVA7|DR1122 from Deinococcus radiodurans (372 aa) FASTA scores: opt: 341, E(): 2e-13, (35.65% identity in 216 aa overlap); P20692|TYRA_BACSU from Bacillus subtilis (372 aa), FASTA scores: opt: 314, E(): 8.6e-12, (27.75% identity in 263 aa overlap); etc. Also similar to Q04983|TYRC_ZYMMO TYRC protein [includes: cyclohexadienyl dehydrogenase and prephenate dehydrogenase activities] from Zymomonas mobilis (293 aa), FASTA scores: opt: 290, E(): 2e-10, (30.15% identity in 239 aa overlap). Equivalent to AAK48225 from Mycobacterium tuberculosis strain CDC1551 (323 aa) but shorter 22 aa.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS42004214201326+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3754|tyrA
MRAAAAAGREVFGYNRSVEGAHGARSDGFDAITDLNQTLTRAAATEALIVLAVPMPALPGMLAHIRKSAPGCPLTDVTSVKCAVLDEVTAAGLQARYVGGHPMTGTAHSGWTAGHGGLFNRAPWVVSVDDHVDPTVWSMVMTLALDCGAMVVPAKSDEHDAAAAAVSHLPHLLAEALAVTAAEVPLAFALAAGSFRDATRVAATAPDLVRAMCEANTGQLAPAADRIIDLLSRARDSLQSHGSIADLADAGHAARTRYDSFPRSDIVTVVIGADKWREQLAAAGRAGGVITSALPSLDSPQ