Gene Rv3765c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Sensor part of a two component regulatory system. |
| Product | Probable two component transcriptional regulatory protein TcrX |
| Comments | Rv3765c, (MTV025.113c), len: 234 aa. Probable tcrX, response regulator of a two-component regulatory system, highly similar to others e.g. Q9ADN7|2SC10A7.24 putative two component system response regulator from Streptomyces coelicolor (271 aa), FASTA scores: opt: 1111, E(): 4.8e-63, (72.3% identity in 231 aa overlap); Q9F161 response regulator from Corynebacterium glutamicum (Brevibacterium flavum) (232 aa), FASTA scores: opt: 692, E(): 1.2e-36, (46.0% identity in 226 aa overlap); Q9KZU5|SCD84.23c putative two-component systen response regulator from Streptomyces coelicolor (248 aa), FASTA scores: opt: 674, E(): 1.7e-35, (44.05% identity in 236 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q50806|Rv1033c|MTCY10G2.16 response regulator homolog (257 aa), FASTA scores: opt: 947, E(): 1e-52, (59.5% identity in 232 aa overlap); P71814|Rv0757|MTCY369.02 PHOP-like protein (247 aa) FASTA scores: opt: 829, E(): 2.8e-45, (54.65% identity in 225 aa overlap); O53894|Rv0981|MTV044.09 (230 aa), FASTA scores: opt: 662, E(): 9e-35, (44.65% identity in 224 aa overlap); and also similar to MTCY31_34; MTCY19H5_20; MTY13628_5; MTCY20G9_17; and to MLCB57_27 from Mycobacterium leprae; and MBY13627_3 from Mycobacterium bovis BCG. Equivalent to AAK48236 from Mycobacterium tuberculosis strain CDC1551 (286 aa) but shorter 52 aa. The N-terminal region is similar to that of other regulatory components of sensory transduction systems. Similar to bacterial regulatory proteins involved in signal transduction. |
| Functional category | Regulatory proteins |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). M. tuberculosis H37Rv tcrXY mutant shows increased virulence in SCID mice (See Parish et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4211080 | 4211784 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3765c|tcrX
MRRADGQPVTVLVVDDEPVLAEMVSMALRYEGWNITTAGDGSSAIAAARRQRPDVVVLDVMLPDMSGLDVLHKLRSENPGLPVLLLTAKDAVEDRIAGLTAGGDDYVTKPFSIEEVVLRLRALLRRTGVTTVDSGAQLVVGDLVLDEDSHEVMRAGEPVSLTSTEFELLRFMMHNSKRVLSKAQILDRVWSYDFGGRSNIVELYISYLRKKIDNGREPMIHTLRGAGYVLKPAR
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Parish T et al. [2003]. Deletion of two-component regulatory systems increases the virulence of Mycobacterium tuberculosis. Mutant Secondary Function
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
