Gene Rv3784 (epiB)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in dTDP-L-rhamnose biosynthesis within the O antigen biosynthesis pathway of lipopolysaccharide biosynthesis [catalytic activity: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H(2)O]. |
| Product | Possible dTDP-glucose 4,6-dehydratase |
| Comments | Rv3784, (MTCY13D12.18), len: 326 aa. Possible dTDP-glucose 4,6-dehydratase, but experimental study shown that the purified protein didn't have dTDP-glucose dehydratase (rmlB) activity (see citation below). Similar to others e.g. Q9YCT1|APE1180 long hypothetical dTDP-glucose 4,6-dehydratase from Aeropyrum pernix (330 aa) FASTA scores: opt: 598, E(): 3.7e-30, (34.9% identity in 315 aa overlap); O27817|MTH1789 dTDP-glucose 4,6-dehydratase from Methanothermobacter thermautotrophicus (336 aa) FASTA scores: opt: 587, E(): 1.8e-29, (34.9% identity in 315 aa overlap); Q9X5W0|GRSE TDP-glucose-4,6-dehydratase homolog from Streptomyces griseus (324 aa), FASTA scores: opt: 583, E(): 3.2e-29, (35.7% identity in 325 aa overlap); Q9K7J7|SPSJ|BH3364 spore coat polysaccharide synthesis (dTDP glucose 4, 6-dehydratase) from Bacillus halodurans (321 aa), FASTA scores: opt: 562, E(): 6.5e-28, (33.0% identity in 318 aa overlap); Q9UZH2|RFBB|PAB0785 dTDP-glucose 4,6-dehydratase from Pyrococcus abyssi (333 aa), FASTA scores: opt: 552, E(): 2.8e-27, (33.95% identity in 318 aa overlap); P27830|RFFG_ECOLI|B3788 dTDP-glucose 4,6-dehydratase from Escherichia coli strain K12 (355 aa), FASTA scores: opt: 401, E(): 7.5e-28, (31.3% identity in 348 aa overlap); etc. But also similar to several UDP-glucose 4-epimerases and other proteins e.g. O59375|PH1742 long hypothetical UDP-glucose 4-epimerase from Pyrococcus horikoshii (306 aa) FASTA scores: opt: 600, E(): 2.6e-30, (34.5% identity in 313 aa overlap); Q9ZGC7|LANH14 NDP-hexose 4,6-dehydratase HOMOLOGfrom Streptomyces cyanogenus (326 aa), FASTA scores: opt: 593, E(): 7.6e-30, (36.45% identity in 321 aa overlap); Q57664|GALE_METJA|MJ0211 putative UDP-glucose 4-epimerase from Methanococcus jannaschii (305 aa) FASTA scores: opt: 575, E(): 9.6e-29, (32.6% identity in 313 aa overlap); etc. Seems to belong to the sugar epimerase family, dTDP-glucose dehydratase subfamily. Note that previously known as epiB. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4230256 | 4231236 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3784|Rv3784
MEILVTGGAGFQGSHLTESLLANGHWVTVLDKSSRNAVRNMQGFRSHDRAAFISGSVTDGQTIDRAVRDHHVVFHLAAHVNVDQSLGDPESFLETNVMGTYRVLEAVRRYRNRLIYVSTCEVYGDGHNLKEGERLDEHAELKPNSPYGASKAAADRLCYSYFRSYGLDVTIVRPFNIFGVRQKAGRFGALIPRLVRQGINGEGLTIFGAGSATRDYLYVSDIVGAYNLVLRTPTLRGQAINFASGKDTRVRDIVEYVADKFGARIEHRDARPGEVQRFPADISLAKSIGFQPQVEIWDGIDRYINWAKDQPQYPYEQDGFSGSSVL
Bibliography
- Ma Y, Stern RJ, Scherman MS, Vissa VD, Yan W, Jones VC, Zhang F, Franzblau SG, Lewis WH and McNeil MR [2001]. Drug targeting Mycobacterium tuberculosis cell wall synthesis: genetics of dTDP-rhamnose synthetic enzymes and development of a microtiter plate-based screen for inhibitors of conversion of dTDP-glucose to dTDP-rhamnose. Product Biochemistry
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
