Gene Rv3790
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Together with DPRE2|Rv3791, catalyzes epimerization of decaprenylphosphoryl ribose (DPR) to decaprenylphosphoryl arabinose (DPA) in arabinan synthesis |
| Product | Decaprenylphosphoryl-beta-D-ribose 2'-oxidase |
| Comments | Rv3790, (MTCY13D12.24), len: 461 aa. DprE1, decaprenylphosphoryl-beta-D-ribose 2'-oxidase, equivalent to Q9CDA4|ML0109 putative FAD-linked oxidoreductase from Mycobacterium leprae (460 aa), FASTA scores: opt: 2722, E(): 1.4e-161, (86.55% identity in 461 aa overlap). Also highly similar to others e.g. Q9KZA4|SC5G8.10c putative oxidoreductase from Streptomyces coelicolor (457 aa), FASTA scores: opt: 1336, E(): 1.7e-75, (47.1% identity in 452 aa overlap); Q98KY4|MLL1265 probable oxidoreductase from Rhizobium loti (Mesorhizobium loti) (449 aa), FASTA scores: opt: 636, E(): 4.9e-32, (36.0% identity in 439 aa overlap); Q9HDX8|SPAPB1A10.12c putative D-arabinono-1,4-lactone oxidase from Schizosaccharomyces pombe (Fission yeast) (461 aa), FASTA scores: opt: 297, E(): 5.6e-11, (23.55% identity in 467 aa overlap); etc. C-terminal end has a high similarity to Q9AQD0 putative oxidoreductase (fragment) from Mycobacterium smegmatis (149 aa) FASTA scores: opt: 901, E(): 6.5e-49, (86.6% identity in 149 aa overlap). Identified as the target of antimicrobial agent 1,3-benzothiazin-4-ones (BTZs) (See Makarov et al., 2009). |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Operon | Rv3790, Rv3791, Rv3792, and Rv3793 are co-transcribed, by Northern blot (See Goude et al., 2008). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4235779 | 4237164 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3790|dprE1
MLSVGATTTATRLTGWGRTAPSVANVLRTPDAEMIVKAVARVAESGGGRGAIARGLGRSYGDNAQNGGGLVIDMTPLNTIHSIDADTKLVDIDAGVNLDQLMKAALPFGLWVPVLPGTRQVTVGGAIACDIHGKNHHSAGSFGNHVRSMDLLTADGEIRHLTPTGEDAELFWATVGGNGLTGIIMRATIEMTPTSTAYFIADGDVTASLDETIALHSDGSEARYTYSSAWFDAISAPPKLGRAAVSRGRLATVEQLPAKLRSEPLKFDAPQLLTLPDVFPNGLANKYTFGPIGELWYRKSGTYRGKVQNLTQFYHPLDMFGEWNRAYGPAGFLQYQFVIPTEAVDEFKKIIGVIQASGHYSFLNVFKLFGPRNQAPLSFPIPGWNICVDFPIKDGLGKFVSELDRRVLEFGGRLYTAKDSRTTAETFHAMYPRVDEWISVRRKVDPLRVFASDMARRLELL
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mikusová K et al. [2005]. Decaprenylphosphoryl arabinofuranose, the donor of the D-arabinofuranosyl residues of mycobacterial arabinan, is formed via a two-step epimerization of decaprenylphosphoryl ribose. Biochemistry
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Goude R et al. [2008]. The critical role of embC in Mycobacterium tuberculosis. Operon
- Makarov V et al. [2009]. Benzothiazinones kill Mycobacterium tuberculosis by blocking arabinan synthesis. Biochemistry
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
