Gene Rv3871 (snm2)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | ESX conserved component EccCb1. ESX-1 type VII secretion system protein. |
| Comments | Rv3871, (MTV027.06), len: 591 aa. EccCb1, esx conserved component, ESX-1 type VII secretion system protein, equivalent to Q9CDD7|ML0052 hypothetical protein from Mycobacterium leprae (597 aa) FASTA scores: opt: 3341, E(): 9.8e-192, (80.85% identity in 596 aa overlap); and O33086|MLCB628.15c hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 3329, E(): 5.1e-191, (80.55% identity in 596 aa overlap). And similar to C-terminal end of others e.g. Q9Z5I2|ML1543|MLCB596.28 possible SPOIIIE-family membrane protein from Mycobacterium leprae (1345 aa), FASTA scores: opt: 601, E(): 5.6e-28, (32.3% identity in 613 aa overlap); O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 977, E(): 2.1e-50, (35.15% identity in 583 aa overlap); Q9L0T6|SCD35.15c putative cell division-related protein from Streptomyces coelicolor (1525 aa), FASTA scores: opt: 414, E(): 9e-17, (27.6% identity in 424 aa overlap);P71068|YUKA YUKA protein from Bacillus subtilis (1207 aa), FASTA scores: opt: 343, E(): 1.3e-12, (25.8% identity in 395 aa overlap); etc. And similar to to C-terminal end of hypothetical proteins from Mycobacterium tuberculosis e.g. O06264|Rv3447c|MTCY77.19c (1236 aa) FASTA scores: opt: 845, E(): 1.5e-42, (35.3% identity in 586 aa overlap); O53689|Rv0284|MTV035.12 (1330 aa) FASTA scores: opt: 646, E(): 1.2e-30, (33.35% identity in 606 aa overlap); O53935|Rv1784|MTV049.06 (932 aa) FASTA scores: opt: 589, E(): 2.1e-27, (33.1% identity in 619 aa overlap); etc. Contains 2 X PS00017 ATP/GTP-binding site motif A (P-loop). Note some similarity (with hypothetical proteins from Mycobacterium tuberculosis and P71068|YUKA) continues in upstream ORF MTV027.05. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h of starvation (see transcriptome citation). mRNA identified by RT-PCR (See Amoudy et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). SCID mice infected with M. tuberculosis H37Rv Rv3871 mutant survive longer than those infected with wild-type; Rv3875 protein detected by Western blot in whole cell extract but not in culture filtrate of mutant (See Hsu et al., 2003). In THP-1 cells, M. tuberculosis H37Rv Rv3871 mutant is attenuated for growth and cytotoxicity; Rv3874 protein levels in cell pellet are comparable to wild-type but are reduced in culture filtrate, by ELISPOT (See Guinn et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4348827 | 4350602 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3871|eccCb1
MTAEPEVRTLREVVLDQLGTAESRAYKMWLPPLTNPVPLNELIARDRRQPLRFALGIMDEPRRHLQDVWGVDVSGAGGNIGIGGAPQTGKSTLLQTMVMSAAATHSPRNVQFYCIDLGGGGLIYLENLPHVGGVANRSEPDKVNRVVAEMQAVMRQRETTFKEHRVGSIGMYRQLRDDPSQPVASDPYGDVFLIIDGWPGFVGEFPDLEGQVQDLAAQGLAFGVHVIISTPRWTELKSRVRDYLGTKIEFRLGDVNETQIDRITREIPANRPGRAVSMEKHHLMIGVPRFDGVHSADNLVEAITAGVTQIASQHTEQAPPVRVLPERIHLHELDPNPPGPESDYRTRWEIPIGLRETDLTPAHCHMHTNPHLLIFGAAKSGKTTIAHAIARAICARNSPQQVRFMLADYRSGLLDAVPDTHLLGAGAINRNSASLDEAVQALAVNLKKRLPPTDLTTAQLRSRSWWSGFDVVLLVDDWHMIVGAAGGMPPMAPLAPLLPAAADIGLHIIVTCQMSQAYKATMDKFVGAAFGSGAPTMFLSGEKQEFPSSEFKVKRRPPGQAFLVSPDGKEVIQAPYIEPPEEVFAAPPSAG
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary Phylogeny
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Hsu T et al. [2003]. The primary mechanism of attenuation of bacillus Calmette-Guerin is a loss of secreted lytic function required for invasion of lung interstitial tissue. Mutant
- Pym AS, Brodin P, Majlessi L, Brosch R, Demangel C, Williams A, Griffiths KE, Marchal G, Leclerc C and Cole ST [2003]. Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis. Gene
- Guinn KM et al. [2004]. Individual RD1-region genes are required for export of ESAT-6/CFP-10 and for virulence of Mycobacterium tuberculosis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Champion PA et al. [2006]. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Biochemistry
- Amoudy HA et al. [2006]. Identification of transcriptionally active open reading frames within the RD1 genomic segment of Mycobacterium tuberculosis. Transcriptome
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
