Gene Rv3877 (snm4)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | ESX conserved component EccD1. ESX-1 type VII secretion system protein. Probable transmembrane protein. |
| Comments | Rv3877, (MTV027.12), len: 511 aa. EccD1, esx conserved component, ESX-1 type VII secretion system protein, probable transmembrane protein, equivalent to Q9CDD9|ML0047 putative membrane protein from Mycobacterium leprae (512 aa), FASTA scores: opt: 2496, E(): 2.8e-140, (74.0% identity in 512 aa overlap); and highly similar, but longer 32 aa, to O33081|MLCB628.10c hypothetical 51.4 KDA protein from Mycobacterium leprae (480 aa), FASTA scores: opt: 2346, E(): 2e-131, (74.15% identity in 480 aa overlap). Shows also similarity with other membrane proteins from Mycobacterium leprae e.g. Q9CBV2|ML1539 probable membrane protein (503 aa), FASTA scores: opt: 318, E(): 2e-11, (22.7% identity in 520 aa overlap). Also similar to various proteins from Mycobacterium tuberculosis e.g. O53944|Rv1795|MTV049.17 putative membrane protein (503 aa), FASTA scores: opt: 391, E(): 9.4e-16, (24.45% identity in 523 aa overlap); O86362|Rv0290|MTV035.18 hypothetical 47.9 KDA protein (472 aa), FASTA scores: opt: 332, E(): 2.8e-12, (28.1% identity in 509 aa overlap); O05457|Rv3887c|MTCY15F10.25 hypothetical 53.2 KDA protein (509 aa), FASTA scores: opt: 167, E(): 0.017, (24.0% identity in 517 aa overlap); etc. Equivalent to AAK48359 from Mycobacterium tuberculosis strain CDC1551 (479 aa) but longer 32 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). SCID mice infected with M. tuberculosis H37Rv Rv3876-Rv3877 mutant survive longer than those infected with wild-type; Rv3875 protein detected by Western blot in whole cell extract but not in culture filtrate of mutant (See Hsu et al., 2003). M. tuberculosis (Erdman) Rv3877 transposon mutant failed to grow in mouse bone marrow-derived macrophages (See MacGurn et al., 2005). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4355007 | 4356542 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3877|eccD1
LSAPAVAAGPTAAGATAARPATTRVTILTGRRMTDLVLPAAVPMETYIDDTVAVLSEVLEDTPADVLGGFDFTAQGVWAFARPGSPPLKLDQSLDDAGVVDGSLLTLVSVSRTERYRPLVEDVIDAIAVLDESPEFDRTALNRFVGAAIPLLTAPVIGMAMRAWWETGRSLWWPLAIGILGIAVLVGSFVANRFYQSGHLAECLLVTTYLLIATAAALAVPLPRGVNSLGAPQVAGAATAVLFLTLMTRGGPRKRHELASFAVITAIAVIAAAAAFGYGYQDWVPAGGIAFGLFIVTNAAKLTVAVARIALPPIPVPGETVDNEELLDPVATPEATSEETPTWQAIIASVPASAVRLTERSKLAKQLLIGYVTSGTLILAAGAIAVVVRGHFFVHSLVVAGLITTVCGFRSRLYAERWCAWALLAATVAIPTGLTAKLIIWYPHYAWLLLSVYLTVALVALVVVGSMAHVRRVSPVVKRTLELIDGAMIAAIIPMLLWITGVYDTVRNIRF
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary Phylogeny
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Pym AS, Brodin P, Majlessi L, Brosch R, Demangel C, Williams A, Griffiths KE, Marchal G, Leclerc C and Cole ST [2003]. Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis. Gene
- Hsu T et al. [2003]. The primary mechanism of attenuation of bacillus Calmette-Guerin is a loss of secreted lytic function required for invasion of lung interstitial tissue. Mutant
- Marmiesse M, Brodin P, Buchrieser C, Gutierrez C, Simoes N, Vincent V, Glaser P, Cole ST and Brosch R [2004]. Macro-array and bioinformatic analyses reveal mycobacterial 'core' genes, variation in the ESAT-6 gene family and new phylogenetic markers for the Mycobacterium tuberculosis complex. Homology
- MacGurn JA et al. [2005]. A non-RD1 gene cluster is required for Snm secretion in Mycobacterium tuberculosis. Mutant
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
