Gene Rv1826
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | The glycine cleavage system catalyses the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein |
| Product | Probable glycine cleavage system H protein GcvH |
| Comments | Rv1826, (MTCY1A11.17c), len: 134 aa. Probable gcvH, glycine cleavage system H protein, highly similar to GCSH_ECOLI|P23884 glycine cleavage system H protein from Escherichia coli (129 aa), FASTA scores: opt: 428, E(): 2.2e-22, (47.8% identity in 134 aa overlap). Equivalent to MLCB1788.37c gcvH from Mycobacterium leprae (78.4% identity in 134 aa overlap). Contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. Belongs to the GcvH family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Operon | Rv1826 and Rv1827 are co-transcribed, by RT-PCR (see Roback et al., 2007). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2071952 | 2072356 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1826|gcvH
VSDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGEVESTKSVSDLYAPISGKVSEVNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALTTLLDAEAYRGTLTE
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Roback P et al. [2007]. A predicted operon map for Mycobacterium tuberculosis. Operon
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
