Gene Rv2316
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. |
| Product | Probable sugar-transport integral membrane protein ABC transporter UspA |
| Comments | Rv2316, (MTCY3G12.18c), len: 290 aa. Probable uspA, sugar-transport integral membrane protein ABC transporter (see citation below), most similar to Q9CBN8|USPA|ML1768 sugar transport integral membrane protein from Mycobacterium leprae (328 aa), FASTA scores: opt: 1593, E(): 1.9e-93, (82.35% identity in 289 aa overlap); and similar to O32940|ML1426|MLCB2052.28 possible sugar transport protein (probable ABC-transport protein, inner membrane component) from Mycobacterium leprae (319 aa), FASTA scores: opt: 600, E(): 9.2e-31, (34.25% identity in 295 aa overlap). Also similar to other proteins involved in transport e.g. Q9X860|SCE134.05c putative binding protein dependent transport protein from Streptomyces coelicolor (327 aa), FASTA scores: opt: 639, E(): 3.2e-33, (40.45% identity in 272 aa overlap); Q9K6N9|BH3689 sugar transport system (permease) from Bacillus halodurans (300 aa), FASTA scores: opt: 590, E(): 3.7e-30, (35.65% identity in 289 aa overlap); etc. |
| Functional category | Cell wall and cell processes |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2588838 | 2589710 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2316|uspA
MRDAPRRRTALAYALLAPSLVGVVAFLLLPILVVVWLSLHRWDLLGPLRYVGLTNWRSVLTDSGFADSLVVTAVFVAIVVPAQTVLGLLAASLLARRLPGTGLFRTLYVLPWICAPLAIAVMWRWIVAPTDGAISTVLGHRIEWLTDPGLALPVVSAVVVWTNVGYVSLFFLAGLMAIPQDIHNAARTDGASAWQRFWRITLPMLRPTMFFVLVTGIISAAQVFDTVYALTGGGPQGSTDLVAHRIYAEAFGAAAIGRASVMAVVLFVILVGATVVQHLYFRRRISYELT
Bibliography
- Braibant M et al. [2000]. The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. Review Secondary
- Titgemeyer F et al. [2007]. A genomic view of sugar transport in Mycobacterium smegmatis and Mycobacterium tuberculosis. Homology
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
