Gene Rv2552c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved at the fourth step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway) [catalytic activity: shikimate + NADP(+) = 5-dehydroshikimate + NADPH]. |
| Product | Probable shikimate 5-dehydrogenase AroE (5-dehydroshikimate reductase) |
| Comments | Rv2552c, (MTCY159.04), len: 269 aa. Probable aroE, shikimate 5-dehydrogenase, equivalent to Q9CCS7|AROE|ML0515 putative shikimate 5-dehydrogenase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1452, E(): 1.8e-77, (81.5% identity in 270 aa overlap). Also highly similar, but longer 101 aa, to Q9KH59|AROE putative shikimate dehydrogenase (fragment) from Mycobacterium marinum (148 aa), FASTA scores: opt: 729, E(): 1.3e-35, (76.35% identity in 148 overlap); Q9F7W3|AROE from Mycobacterium ulcerans (148 aa), FASTA scores: opt: 718, E(): 5.9e-35, (75.7% identity in 148 aa overlap). And also similar to to others e.g. Q9KXQ2|AROE from Streptomyces coelicolor (255 aa), FASTA scores: opt: 572, E(): 2.8e-26, (43.4% identity in 251 aa overlap); Q98DY3|MLR4492 from Rhizobium loti (Mesorhizobium loti) (280 aa), FASTA scores: opt: 385, E(): 2.2e-15, (34.85% identity in 284 aa overlap); P74591|AROE_SYNY3|SLR1559 from Synechocystis sp. strain PCC 6803 (290 aa), FASTA scores: opt: 347, E(): 3.7e-13, (30.9% identity in 275 aa overlap); P15770|AROE_ECOLI|B3281 from Escherichia coli strain K12 (272 aa), FASTA scores: opt: 230, E(): 7.7e-08, (29.5% identity in 251 aa overlap); etc. Belongs to the shikimate dehydrogenase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019).Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2871206 | 2872015 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2552c|aroE
MSEGPKKAGVLGSPIAHSRSPQLHLAAYRALGLHDWTYERIECGAAELPVVVGGFGPEWVGVSVTMPGKFAALRFADERTARADLVGSANTLVRTPHGWRADNTDIDGVAGALGAAAGHALVLGSGGTAPAAVVGLAELGVTDITVVARNSDKAARLVDLGTRVGVATRFCAFDSGGLADAVAAAEVLVSTIPAEVAAGYAGTLAAIPVLLDAIYDPWPTPLAAAVGSAGGRVISGLQMLLHQAFAQVEQFTGLPAPREAMTCALAALD
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
