Gene Rv2737c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in regulation of nucleotide excision repair, in genetic recombination, and in induction of the sos response. Endonuclease which can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAS. It interacts with LEXA|Rv2720 causing its activation and leading to its autocatalytic cleavage. |
| Product | RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)]. |
| Comments | Rv2737c, (MTV002.02c), len: 790 aa. RecA, recombinase a (see citations below), equivalent to Q59560|RECA_MYCSM RECA protein from Mycobacterium smegmatis (349 aa), FASTA scores: opt: 1495, E(): 1.9e-79, (93.15% identity in 249 aa overlap); and P35901|RECA_MYCLE|ML0987 RECA protein from Mycobacterium leprae (711 aa), FASTA scores: opt: 1217, E(): 4.5e-63, (46.7% identity in 814 aa overlap). Also highly similar to many e.g. Q9REV6|RECA_AMYMD from Amycolatopsis mediterranei (Nocardia mediterranei) (348 aa), FASTA scores: opt: 1450, E(): 7.6e-77, (89.25% identity in 251 aa overlap); P42442|RECA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (376 aa), FASTA scores: opt: 1355, E(): 2.6e-71, (76.55% identity in 273 aa overlap); P41054|RECA_STRAM from Streptomyces ambofaciens (372 aa), FASTA scores: opt: 1347, E(): 7.6e-71, (82.1% identity in 246 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00321 recA signature, and PS00881 Protein splicing signature. Belongs to the RecA family. This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Belongs to the homing endonuclease family in the intein section. |
| Functional category | Information pathways |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | mRNA identified by Microarray analysis (see Davis et al., 2002) and, in Mycobacterium smegmatis, by RT-PCR (see Papavinasasundaram et al., 1997). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Slow growth mutant by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3049052 | 3051424 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2737c|recA
MTQTPDREKALELAVAQIEKSYGKGSVMRLGDEARQPISVIPTGSIALDVALGIGGLPRGRVIEIYGPESSGKTTVALHAVANAQAAGGVAAFIDAEHALDPDYAKKLGVDTDSLLVSQPDTGEQALEIADMLIRSGALDIVVIDSVAALVPRAELEGEMGDSHVGLQARLMSQALRKMTGALNNSGTTAIFINQLRDKIGVMFGSPETTTGGKALKFYASVRMDVRRVETLKDGTNAVGNRTRVKVVKNKCLAEGTRIFDPVTGTTHRIEDVVDGRKPIHVVAAAKDGTLHARPVVSWFDQGTRDVIGLRIAGGAIVWATPDHKVLTEYGWRAAGELRKGDRVAQPRRFDGFGDSAPIPADHARLLGYLIGDGRDGWVGGKTPINFINVQRALIDDVTRIAATLGCAAHPQGRISLAIAHRPGERNGVADLCQQAGIYGKLAWEKTIPNWFFEPDIAADIVGNLLFGLFESDGWVSREQTGALRVGYTTTSEQLAHQIHWLLLRFGVGSTVRDYDPTQKRPSIVNGRRIQSKRQVFEVRISGMDNVTAFAESVPMWGPRGAALIQAIPEATQGRRRGSQATYLAAEMTDAVLNYLDERGVTAQEAAAMIGVASGDPRGGMKQVLGASRLRRDRVQALADALDDKFLHDMLAEELRYSVIREVLPTRRARTFDLEVEELHTLVAEGVVVHNCSPPFKQAEFDILYGKGISREGSLIDMGVDQGLIRKSGAWFTYEGEQLGQGKENARNFLVENADVADEIEKKIKEKLGIGAVVTDDPSNDGVLPAPVDF
Bibliography
- Davis EO et al. [1991]. Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product. Product Regulation
- Davis EO et al. [1992]. Protein splicing in the maturation of M. tuberculosis recA protein: a mechanism for tolerating a novel class of intervening sequence. Regulation
- McFadden J [1996]. Recombination in mycobacteria. Review
- Kumar RA et al. [1996]. Functional characterization of the precursor and spliced forms of RecA protein of Mycobacterium tuberculosis. Mutant Product
- Papavinasasundaram KG et al. [1997]. Mycobacterial recA is cotranscribed with a potential regulatory gene called recX. Homolog Sequence Transcriptome
- Mulder MA et al. [1997]. Mycobacterial promoters. Review Regulation
- Durbach SI et al. [1997]. SOS induction in mycobacteria: analysis of the DNA-binding activity of a LexA-like repressor and its role in DNA damage induction of the recA gene from Mycobacterium smegmatis. Product Regulation
- Mizrahi V et al. [1998]. DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence? Secondary Function
- Datta S et al. [2000]. Crystal structures of Mycobacterium tuberculosis RecA and its complex with ADP-AlF(4): implications for decreased ATPase activity and molecular aggregation. Structure
- Papavinasasundaram KG et al. [2001]. Slow induction of RecA by DNA damage in Mycobacterium tuberculosis. Product
- Brooks PC, Movahedzadeh F and Davis EO [2001]. Identification of some DNA damage-inducible genes of Mycobacterium tuberculosis: apparent lack of correlation with LexA binding. Function
- Davis EO et al. [2002]. Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis. Sequence Regulation Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Datta S et al. [2003]. Structural studies on MtRecA-nucleotide complexes: insights into DNA and nucleotide binding and the structural signature of NTP recognition. Structure
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Van Roey P et al. [2007]. Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue. Structure
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
