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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionCatalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate
ProductProbable thymidylate synthase ThyX (ts) (TSase)
CommentsRv2754c, (MTV002.19c), len: 250 aa. Probable thyX, thymidylate synthase, highly similar to Q9CBW3|YF14_MYCLE|ML1514 thymidylate synthase from Mycobacterium leprae (254 aa), FASTA scores: opt: 1351, E(): 1e-84, (81.5% identity in 254 aa overlap). Also highly similar to several others e.g P40111|THYX_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (250 aa), FASTA scores: opt: 1080, E(): 9.8e-67, (62.85% identity in 245 aa overlap); Q05259|THYX_BPML5 Probable thymidylate synthase from Mycobacteriophage L5 (243 aa), FASTA scores: opt: 610, E(): 3.2e-34, (49.55% identity in 220 aa overlap); etc. Contains Pfam match to entry PF02511 Thymidylate synthase complementing protein. Belongs to the THY1 family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantEssential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS30671933067945-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2754c|thyX
VAETAPLRVQLIAKTDFLAPPDVPWTTDADGGPALVEFAGRACYQSWSKPNPKTATNAGYLRHIIDVGHFSVLEHASVSFYITGISRSCTHELIRHRHFSYSQLSQRYVPEKDSRVVVPPGMEDDADLRHILTEAADAARATYSELLAKLEAKFADQPNAILRRKQARQAARAVLPNATETRIVVTGNYRAWRHFIAMRASEHADVEIRRLAIECLRQLAAVAPAVFADFEVTTLADGTEVATSPLATEA