Gene Rv2847c (cysG2)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the biosynthesis of siroheme and cobalamin [catalytic activity: 2 S-adenosyl-L-methionine + uroporphyrin III = 2 S-adenosyl-L-homocysteine + sirohydrochlorin]. SAM-dependent methyl transferase that methylates uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It catalyzes also the conversion of precorrin-2 into siroheme (consisting of an oxidation and FE(2+) chelation). |
| Product | Possible multifunctional enzyme siroheme synthase CysG: uroporphyrin-III C-methyltransferase (urogen III methylase) (SUMT) (uroporphyrinogen III methylase) (UROM) + precorrin-2 oxidase + ferrochelatase |
| Comments | Rv2847c, (MTCY24A1.10), len: 405 aa. Possible cysG, multifunctional enzyme, siroheme synthase containing uroporphyrin-III c-methyltransferase, precorrin-2 oxidase and ferrochelatase. C-terminus highly similar to many uroporphyrin-III c-methyltransferases e.g. Q51720|COBA uroporphyrinogen III methyltransferase from Propionibacterium freudenreichii (257 aa), FASTA scores: opt: 776, E(): 1.5e-39, (48.95% identity in 243 aa overlap); Q9HMY4|UROM|VNG2331G S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Halobacterium sp. strain NRC-1 (246 aa), FASTA scores: opt: 704, E(): 3.1e-35, (49.4% identity in 245 aa overlap); P42437|NASF_BACSU|NASBE uroporphyrin-III C-methyltransferase from Bacillus subtilis (483 aa), FASTA scores: opt: 610, E(): 2.4e-29, (42.1% identity in 240 aa overlap); etc. And highly similar over entire length to other proteins e.g. Q9L1C9|SCL11.09c uroporphyrinogen III methyltransferase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1481, E(): 5.6e-82, (58.45% identity in 409 aa overlap); Q9I0M7|CYSG|PA2611 siroheme synthase from Pseudomonas aeruginosa (465 aa), FASTA scores: opt: 609, E(): 2.7e-29, (34.7% identity in 444 aa overlap); P11098|CYSG_ECOLI|B3368|Z4729|ECS4219 siroheme synthase from Escherichia coli stains O157:H7 and K12 (457 aa), FASTA scores: opt: 543, E(): 9.1e-27, (31.3% identity in 450 aa overlap); etc. Belongs to a family that groups SUMT, CYSG, CBIF/COBM and CBIL/COBI. Note that previously known as cysG2. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell wall and cell membrane fractions of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3154654 | 3155871 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2847c|cysG
VTENPYLVGLRLAGKKVVVVGGGTVAQRRLPLLIASGADVHVIAPSVTPAVEAMDQITLSVRDYRDGDLDGAWYAIAATDDARVNVAVVAEAERRRIFCVRADIAVEGTAVTPASFSYAGLSVGVLAGGEHRRSAAIRSAIREALQQGVITAQSSDVLSGGVALVGGGPGDPELITVRGRRLLAQADVVVADRLAPPELLAELPPHVEVIDAAKIPYGRAMAQDAINAVLIERARSGNFVVRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTSAIAVPAMAGVPVTHRAMTHEFVVVSGHLAPGHPESLVNWDALAALTGTIVLLMAVERIELFVDVLLKGGRTADTPVLVVQHGTTAAQQTLRATLADTPEKVRAAGIRPPAIIVIGAVVGLSGVRGLNNS
Bibliography
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Proteogenomic analysis of polymorphisms and gene annotation divergences in prokaryotes using a clustered mass spectrometry-friendly database. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
