Gene Rv2894c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Participates in the site-specific recombination. Acts by catalyzing the cutting and rejoining of the recombinating DNA molecules. Acts jointly with XERD. |
| Product | Probable integrase/recombinase XerC |
| Comments | Rv2894c, (MTCY274.25c), len: 298 aa. Probable xerC, integrase/recombinase, equivalent to Q9CBU0|XERC|ML1600|MLCB250.62 integrase/recombinase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1624, E(): 2e-97, (85.15% identity in 296 aa overlap). Also highly similar to others integrases/recombinases (generally xerC and xerD) e.g. Q9HTS4|SSS|PA5280 site-specific recombinase from Pseudomonas aeruginosa (303 aa), FASTA scores: opt: 660, E(): 3.2e-35, (41.8% identity in 299 aa overlap); Q9HXQ6|XERD|PA3738 integrase/recombinase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 656, E(): 5.7e-35, (40.05% identity in 297 aa overlap); Q9KCP0|BH1529 integrase/recombinase from Bacillus halodurans (299 aa), FASTA scores: opt: 645, E(): 2.9e-34, (37.35% identity in 300 aa overlap); etc. Also similar to O33200|Rv1701|MTCI125.23 integrase/recombinase from Mycobacterium tuberculosis (311 aa), FASTA scores: opt: 646, E(): 2.6e-34, (43.1% identity in 304 aa overlap). Belongs to the 'phage' integrase family. |
| Functional category | Insertion seqs and phages |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | DNA microarrays show lower level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3203394 | 3204290 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2894c|xerC
VQAILDEFDEYLALQCGRSVHTRRAYLGDLRSLFAFLADRGSSLDALTLSVLRSWLAATAGAGAARTTLARRTSAVKAFTAWAVRRGLLAGDPAARLQVPKARRTLPAVLRQDQALRAMAAAESGAEQGDPLALRDRLIVELLYATGIRVSELCGLDVDDIDTGHRLVRVLGKGNKQRTVPFGQPAADALHAWLVDGRRALVTAESGHALLLGARGRRLDVRQARTAVHQTVAAVDGAPDMGPHGLRHSAATHLLEGGADLRVVQELLGHSSLATTQLYTHVAVARLRAVHERAHPRA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
