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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in cell wall formation. Along with alanine racemase, it MAKES up the D-alanine branch of the peptidoglycan biosynthetic route. [catalytic activity: ATP + D-alanine + D-alanine = ADP + phosphate + D-alanyl-D-alanine].
ProductProbable D-alanine--D-alanine ligase DdlA (D-alanylalanine synthetase) (D-ala-D-ala ligase)
CommentsRv2981c, (MTCY349.06), len: 373 aa. Probable ddlA (alternate gene name: ddl), D-alanine--D-alanine ligase a (see citation below), equivalent to Q9CBS0|Q9CBS0 D-alanine-D-alanine ligase a from Mycobacterium leprae (384 aa), FASTA scores: opt: 2001, E(): 2.4e-115, (81.75% identity in 367 aa overlap); and Q9ZGN0|DDL_MYCSM D-alanine--D-alanine ligase from Mycobacterium smegmatis (373 aa), FASTA scores: opt: 1934, E(): 3.1e-111, (77.95% identity in 372 aa overlap). Also highly similar to others e.g. Q9ZBR9|DDL_STRCO from Streptomyces coelicolor (389 aa), FASTA scores: opt: 1187, E(): 2.2e-65, (52.0% identity in 379 aa overlap); P15051|DDLA_SALTY from Salmonella typhimurium and Salmonella typhi (363 aa), FASTA scores: opt: 946, E(): 1.3e-50, (44.5% identity in 364 aa overlap); P23844|DDLA_ECOLI|DDLA|B0381|Z0477|ECS0431 from Escherichia coli strain O157:H7 and K12 (364 aa), FASTA scores: opt: 938, E(): 3.9e-50, (43.55% identity in 363 aa overlap); etc. Contains PS00843 D-alanine--D-alanine ligase signature 1. Belongs to the D-alanine--D-alanine ligase family.
Functional categoryCell wall and cell processes
ProteomicsIdentified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantEssential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS33367963337917-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2981c|ddlA
VSANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTDANPDALTITNRELPQVKSGSGTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLAADGLPVGAYAVLRPPRSTLHRQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELECGVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQLAIRAFAAIDCRGLARVDFFLTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLATMIETTLARGVGLH