Gene Rv3198c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in nucleotide excision repair. Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the postincision events of nucleotide excision repair and methyl-directed mismatch repair. |
| Product | Probable ATP-dependent DNA helicase II UvrD2 |
| Comments | Rv3198c, (MTV014.42c), len: 700 aa. Probable UvrD2, ATP dependent DNA helicase II (see citation below), equivalent to P53528|UVRD_MYCLE|VRD|UVRD2|ML0637|B1937_F1_27 probable DNA helicase II homolog from Mycobacterium leprae (717 aa), FASTA scores: opt: 3749, E(): 0, (82.85% identity in 706 aa overlap); and C-terminal half (466-700 aa) corresponds to Q49764|RECQ|B1937_F2_66 putative DNA helicase RECQ (242 aa), FASTA scores: opt: 1267, E(): 1.4e-69, (82.5% identity in 234 aa overlap); products of two adjacent ORFS in Mycobacterium leprae. Also similar to other DNA helicases e.g. Q9FCK0|2SC3B6.12 from Streptomyces coelicolor (785 aa), FASTA scores: opt: 1687, E(): 1.2e-94, (52.05% identity in 728 aa overlap); P71561|CRA|IVRD|Rv0949|MT0976|MTCY10D7.25c ATP-dependent DNA helicase PCRA from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 715, E(): 1e-35, (34.1% identity in 710 aa overlap); Q9CD72|PCRA_MYCLE|UVRD|ML0153 ATP-dependent DNA helicase PCRA from Mycobacterium leprae (778 aa), FASTA scores: opt: 687, E(): 5.1e-34, (32.0% identity in 719 aa overlap); O83991|TP1028 DNA helicase II (UVRD) from Treponema pallidum (670 aa), FASTA scores: opt: 652, E(): 6e-32, (30.25% identity in 671 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UVRD subfamily of helicases. |
| Functional category | Information pathways |
| Proteomics | Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene domain for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al.,2003). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3569109 | 3571211 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3198c|uvrD2
MSIASDPLIAGLDDQQREAVLAPRGPVCVLAGAGTGKTRTITHRIASLVASGHVAAGQVLAVTFTQRAAGEMRSRLRALDAAARTGSGVGAVQALTFHAAAYRQLRYFWSRVIADTGWQLLDSKFAVVARAASRTRLHASTDDVRDLAGEIEWAKASLIGPEEYVTAVAAARRDPPLDAAQIAAVYSEYEALKARGDGVTLLDFDDLLLHTAAAIENDAAVAEEFQDRYRCFVVDEYQDVTPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPRFLLDFSRRFPDAAVVRLERDYRSTPQVVSLANRVIAAARGRVAGSKLRLSGQREPGPVPSFHEHSDEPAEAATVAASIARLIASGTPPSEVAILYRVNAQSEVYEEALTQAGIAYQVRGGEGFFNRQEIKQALLALQRVSERDTDAALSDVVRAVLAPLGLTAQPPVGTRARERWEALTALAELVDDELAQRPALQLPGLLAELRRRAEARHPPVVQGVTLASLHAAKGLEWDAVFLVGLADGTLPISHALAHGPNSEPVEEERRLLYVGITRARVHLALSWALSRSPGGRQSRKPSRFLNGIAPQTRADPVPGTSRRNRGAAARCRICNNELNTSAAVMLRRCETCAADVDEELLLQLKSWRLSTAKEQNVPAYVVFTDNTLIAIAELLPTDDAALIAIPGIGARKLEQYGSDVLQLVRGRT
Bibliography
- Mizrahi V et al. [1998]. DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence? Secondary Function
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
