Gene Rv3260c (whmD)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Probable transcriptional regulatory protein WhiB-like WhiB2 |
| Comments | Rv3260c, (MTV015.05c), len: 89 aa. Probable whiB2 (alternate gene name: whmD), WhiB-like regulatory protein (see Hutter & Dick 1999), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to Q9CCK0|WHIB2|ML0760 putative transcriptional regulator from Mycobacterium leprae (89 aa), FASTA scores: opt: 550, E(): 6.1e-31, (85.4% identity in 89 aa overlap). Also similar to others e.g. Q9S426 WHMD regulatory protein (see Gomez & Bishai 2000) from Mycobacterium smegmatis (129 aa), FASTA scores: opt: 488, E(): 1.4e-26, (83.55% identity in 85 aa overlap); Q06387|WHIB-STV WHIB-STV protein from Streptomyces griseocarneus (87 aa), FASTA scores: opt: 443, E(): 1.2e-23, (74.7% identity in 83 aa overlap); Q05429|WHIB|WHIB1 transcription-like factor WhiB from Streptomyces aureofaciens (87 aa), FASTA scores: opt: 442, E(): 1.3e-23, (74.7% identity in 83 aa overlap); etc. Equivalent to AAK47700 WhiB-related protein from Mycobacterium tuberculosis strain CDC1551 (123 aa) but shorter 34 aa. Also similar to other Mycobacterium tuberculosis proteins: MTCY07D11.07c (45.1% identity in 71 aa overlap) and MTCY78.13c (37.4% identity in 91 aa overlap). Start chosen by homology but ORF continues to ATG upstream at 3754. |
| Functional category | Regulatory proteins |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 4h, 24h and 96h of starvation (see Betts et al., 2002). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019).Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3639872 | 3640141 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3260c|whiB2
LVPEAPAPFEEPLPPEATDQWQDRALCAQTDPEAFFPEKGGSTREAKKICMGCEVRHECLEYALAHDERFGIWGGLSERERRRLKRGII
Bibliography
- Hutter B and Dick T [1999]. Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor. Homolog Mutant
- Gomez JE et al. [2000]. whmD is an essential mycobacterial gene required for proper septation and cell division. Homolog Mutant
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
