Gene Rv3266c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in dTDP-L-rhamnose biosynthesis: converts dTDP-6-deoxy-L-lyxo-4-hexulose to dTDP-L-rhamnose with the concomitant oxidation of NADPH to NADP+ [catalytic activity: dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH = dTDP-L-rhamnose + NADP+]. |
| Product | dTDP-6-deoxy-L-lyxo-4-hexulose reductase RmlD (dTDP-rhamnose modification protein) (dTDP-rhamnose biosynthesis protein) (dTDP-rhamnose synthase) |
| Comments | Rv3266c, (MTCY71.06c), len: 304 aa. RmlD, dTDP-6-deoxy-L-lyxo-4-hexulose reductase (dTDP-rhamnose modification protein) (see citations below), highly similar to Q9CCK8 putative dTDP-rhamnose modification protein from Mycobacterium leprae (311 aa), FASTA scores, opt: 1440, E(): 1.1e-78, (74.7% identity in 312 aa overlap); and similar to several dTDP-4-dehydrorhamnose reductase e.g. STRL_STRGR|P29781 from Streptomyces griseus (304 aa), FASTA scores, opt: 788, E(): 0, (47.4% identity in 304 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. smegmatis knockout shows rmlD is essential for growth (See Ma et al., 2002). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3646895 | 3647809 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3266c|rmlD
MAGRSERLVITGAGGQLGSHLTAQAAREGRDMLALTSSQWDITDPAAAERIIRHGDVVINCAAYTDVDGAESNEAVAYAVNATGPQHLARACARVGARLIHVSTDYVFDGDFGGAEPRPYEPTDETAPQGVYARSKLAGEQAVLAAFPEAAVVRTAWVYTGGTGKDFVAVMRRLAAGHGRVDVVDDQTGSPTYVADLAEALLALADAGVRGRVLHAANEGVVSRFGQARAVFEECGADPQRVRPVSSAQFPRPAPRSSYSALSSRQWALAGLTPLRHWRSALATALAAPANSTSIDRRLPSTRD
Bibliography
- Giraud MF et al. [1999]. Overexpression, purification, crystallization and preliminary structural study of dTDP-6-deoxy-L-lyxo-4-hexulose reductase (RmlD), the fourth enzyme of the dTDP-L-rhamnose synthesis pathway, from Salmonella enterica serovar Typhimurium. Homolog Product Structure
- Hoang TT et al. [1999]. Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of mycobacterium tuberculosis RmlD and pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI. Product
- Belanger AE and Inamine JM [2000]. Review
- Ma Y, Stern RJ, Scherman MS, Vissa VD, Yan W, Jones VC, Zhang F, Franzblau SG, Lewis WH and McNeil MR [2001]. Drug targeting Mycobacterium tuberculosis cell wall synthesis: genetics of dTDP-rhamnose synthetic enzymes and development of a microtiter plate-based screen for inhibitors of conversion of dTDP-glucose to dTDP-rhamnose. Product Biochemistry Function
- Ma Y et al. [2002]. Formation of dTDP-rhamnose is essential for growth of mycobacteria. Homolog Mutant Transcriptome
- Valvano MA, Messner P and Kosma P [2002]. Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Review Function
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
