Gene Rv3295
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Probable transcriptional regulatory protein (probably TetR-family) |
| Comments | Rv3295, (MTCY71.35), len: 221 aa. Probable transcriptional regulator TetR-family, equivalent to Q9CCL4|ML0717 putative TetR-family transcriptional regulator from Mycobacterium leprae (223 aa), FASTA scores: opt: 1260, E(): 7.2e-75, (85.45% identity in 220 aa overlap). Also highly similar to other streptomyces regulators e.g. Q9RD77|SCF43.11 from Streptomyces coelicolor (205 aa), FASTA scores: opt: 442, E(): 9.8e-22, (38.6% identity in 202 aa overlap); Q9RKY8|SC6D7.09 from Streptomyces coelicolor (220 aa), FASTA scores: opt: 215, E(): 5.9e-07, (31.85% identity in 135 aa overlap); Q9L0U5|SCD35.06 from Streptomyces coelicolor (240 aa), FASTA scores: opt: 214, E(): 7.4e-07, (28.2% identity in 156 aa overlap); etc. Similar to the TetR/AcrR family of transcriptional regulators. Contains potential helix-turn-helix motif at aa 33-54 (+4.42 SD). |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis; transcription repressed at low pH in vitro conditions, which may mimic an environmental signal encountered by phagocytosed bacteria (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3676066 | 3676731 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3295|Rv3295
MATARRRLSPQDRRAELLALGAEVFGKRPYDEVRIDEIAERAGVSRALMYHYFPDKRAFFAAVVKDEADRLYAATNKAPAPGMTMFEEIRTGVLAYMAYHQQNPEAAWAAYVGLGRSDPVLLGIDDEAKNRQMEHIMSRIAEVVSGIDRDNTLDPEVERDLRVIIHGWLAFTFELCRQRIMDPSTDAERLADACAHALLDAISRLPQIPAELADAMATARM
Bibliography
- Fisher MA, Plikaytis BB and Shinnick TM [2002]. Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes. Transcriptome Regulation
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
