Gene Rv3423c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Provides the D-alanine required for cell wall biosynthesis. Transforms L-alanine to D-alanine [catalytic activity: L-alanine = D-alanine] |
| Product | Alanine racemase Alr |
| Comments | Rv3423c, (MTCY78.06), len: 408 aa. Alr, alanine racemase, equivalent to P38056|ALR_MYCLE|ML0375|B229_C3_243 alanine racemase from Mycobacterium leprae (388 aa), FASTA scores: opt: 2160, E(): 2.3e-124, (84.35% identity in 384 aa overlap). Also highly similar to other alanine racemases e.g. Q9L888|ALR_MYCAV from Mycobacterium avium (391 aa), FASTA scores: opt: 2103, E(): 6.8e-121, (83.6% identity in 384 aa overlap); P94967|ALR_MYCSM from Mycobacterium smegmatis (389 aa), FASTA scores: opt: 1721, E(): 1.3e-97, (67.25% identity in 385 aa overlap); O86786|ALR_STRCO|SC6G4.23 from Streptomyces coelicolor (391 aa), FASTA scores: opt: 1041, E(): 3.7e-56, (47.65% identity in 380 aa overlap); etc. Contains Pfam entry PF00842 Alanine racemase. Belongs to the alanine racemase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3840194 | 3841420 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3423c|alr
VKRFWENVGKPNDTTDGRGTTSLAMTPISQTPGLLAEAMVDLGAIEHNVRVLREHAGHAQLMAVVKADGYGHGATRVAQTALGAGAAELGVATVDEALALRADGITAPVLAWLHPPGIDFGPALLADVQVAVSSLRQLDELLHAVRRTGRTATVTVKVDTGLNRNGVGPAQFPAMLTALRQAMAEDAVRLRGLMSHMVYADKPDDSINDVQAQRFTAFLAQAREQGVRFEVAHLSNSSATMARPDLTFDLVRPGIAVYGLSPVPALGDMGLVPAMTVKCAVALVKSIRAGEGVSYGHTWIAPRDTNLALLPIGYADGVFRSLGGRLEVLINGRRCPGVGRICMDQFMVDLGPGPLDVAEGDEAILFGPGIRGEPTAQDWADLVGTIHYEVVTSPRGRITRTYREAENR
Bibliography
- Strych U et al. [2001]. Characterization of the alanine racemases from two mycobacteria. Product Biochemistry
- Chacon O et al. [2002]. Mycobacterium smegmatis D-Alanine Racemase Mutants Are Not Dependent on D-Alanine for Growth. Homolog Mutant Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Feng Z et al. [2003]. Roles of Mycobacterium smegmatis D-alanine:D-alanine ligase and D-alanine racemase in the mechanisms of action of and resistance to the peptidoglycan inhibitor D-cycloserine. Gene
- LeMagueres P et al. [2005]. The 1.9 A crystal structure of alanine racemase from Mycobacterium tuberculosis contains a conserved entryway into the active site. Structure
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
