Gene Rv3598c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in translation [catalytic activity: ATP + L-lysine + tRNA(LYS) = AMP + pyrophosphate + L-lysyl-tRNA(LYS)]. |
| Product | Lysyl-tRNA synthetase 1 LysS (lysine--tRNA ligase 1) (LysRS 1) (lysine translase) |
| Comments | Rv3598c, (MTCY07H7B.24), len: 505 aa. Probable lysS, lysyl-tRNA synthetase 1, equivalent to P46861|SYK_MYCLE|LYSS|ML0233 lysyl-tRNA synthetase from Mycobacterium leprae (507 aa), FASTA scores: opt: 2835, E(): 4.5e-172, (85.45% identity in 501 aa overlap); and similar with C-terminal part of Q9CC23|LYSX|ML1393 C-term lysyl-tRNA synthase from Mycobacterium leprae (1039 aa) FASTA scores: opt: 1257, E(): 7.6e-72, (44.55% identity in 505 aa overlap). Also similar to others e.g. P37477|SYK_BACSU|LYSS from Bacillus subtilis (499 aa) FASTA scores: opt: 1294, E(): 1.9e-74, (42.35% identity in 498 aa overlap); Q9RHV9|SYK_BACST|LYSS from Bacillus stearothermophilus (494 aa), FASTA scores: opt: 1258, E(): 3.5e-72, (41.15% identity in 498 aa overlap); Q9PEB6|SYK_XYLFA|LYSS|XF1112 from Xylella fastidiosa (506 aa), FASTA scores: opt: 1228, E(): 2.9e-70, (43.05% identity in 495 aa overlap); etc. Also similar to P94974|SYK2_MYCTU|LYSS2|LYSX|Rv1640c|MTCY06H11.04c lysyl-tRNA synthetase 2 from Mycobacterium tuberculosis (1172 aa), FASTA scores: opt: 1295, E(): 3.3e-74, (45.65% identity in 506 aa overlap). Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. |
| Functional category | Information pathways |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4041423 | 4042940 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3598c|lysS
VSAADTAEDLPEQFRIRRDKRARLLAQGRDPYPVAVPRTHTLAEVRAAHPDLPIDTATEDIVGVAGRVIFARNSGKLCFATLQDGDGTQLQVMISLDKVGQAALDAWKADVDLGDIVYVHGAVISSRRGELSVLADCWRIAAKSLRPLPVAHKEMSEESRVRQRYVDLIVRPEARAVARLRIAVVRAIRTALQRRGFLEVETPVLQTLAGGAAARPFATHSNALDIDLYLRIAPELFLKRCIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTRELIQEVADEAIGTRQLPLPDGSVYDIDGEWATIQMYPSLSVALGEEITPQTTVDRLRGIADSLGLEKDPAIHDNRGFGHGKLIEELWERTVGKSLSAPTFVKDFPVQTTPLTRQHRSIPGVTEKWDLYLRGIELATGYSELSDPVVQRERFADQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGIDRLLMSLTGLSIRETVLFPIVRPHSN
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
