Gene Rv3627c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown (possibly involved in cell wall biosynthesis). |
| Product | Conserved protein |
| Comments | Rv3627c, (MTCY15C10.25), len: 461 aa. Conserved ala-rich protein which may have cleavable signal peptide at N-terminal end. Equivalent to O69539|MLCB2548.20c|ML0211 hypothetical 47.2 KDA protein from Mycobacterium leprae (461 aa), FASTA scores: opt: 2295, E(): 3.5e-116, (76.2% identity in 462 aa overlap); and C-terminal end shows similarity with O05758|MLCB5.28c hypothetical 24.1 KDA protein from Mycobacterium leprae (225 aa), FASTA scores: opt: 268, E(): 1.8e-07, (32.25% identity in 220 aa overlap). Also similar (or with similarity) to various proteins (notably penicillin binding proteins) e.g. Q9X8I8|SCE9.15c hypothetical 45.9 KDA protein from Streptomyces coelicolor (459 aa) FASTA scores: opt: 707, E(): 8.3e-31, (35.75% identity in 439 aa overlap); Q9Z541|SC9B2.18c putative carboxypeptidase from Streptomyces coelicolor (451 aa), FASTA scores: opt: 450, E(): 5.3e-17, (31.75% identity in 469 aa overlap); Q9JVV4|NMA0665 putative peptidase from Neisseria meningitidis (serogroup A) (or Q9JY10|NMB1797 from serogroup B) (469 aa), FASTA scores: opt: 269, E(): 3e-07, (26.15% identity in 463 aa overlap); O85665|PBP3 penicillin binding protein 3 from Neisseria gonorrhoeae (469 aa), FASTA scores: opt: 265, E(): 4.9e-07, (31.85% identity in 201 aa overlap); P45161|PBP4_HAEIN|DACB|HI1330 penicillin-binding protein 4 precursor/peptidase (479 aa) FASTA scores: opt: 230, E(): 3.8e-05, (27.9% identity in 394 aa overlap); P24228|PBP4_ECOLI|DACB|B3182 penicillin-binding protein 4 precursor from Escherichia coli strain K12 (477 aa), FASTA scores: opt: 166, E(): 0.1, (28.2% identity in 408 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4065900 | 4067285 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3627c|Rv3627c
MGPTRWRKSTHVVVGAAVLAFVAVVVAAAALVTTGGHRAGVRAPAPPPRPPTVKAGVVPVADTAATPSAAGVTAALAVVAADPDLGKLAGRITDALTGQELWQRLDDVPLVPASTNKILTAAAALLTLDRQARISTRVVAGGQNPQGPVVLVGAGDPTLSAAPPGQDTWYHGAARIGDLVEQIRRSGVTPTAVQVDASAFSGPTMAPGWDPADIDNGDIAPIEAAMIDAGRIQPTTVNSRRSRTPALDAGRELAKALGLDPAAVTIASAPAGARQLAVVQSAPLIQRLSQMMNASDNVMAECIGREVAVAINRPQSFSGAVDAVTSRLNTAHIDTAGAALVDSSGLSLDNRLTARTLDATMQAAAGPDQPALRPLLDLLPIAGGSGTLGERFLDAATDQGPAGWLRAKTGSLTAINSLVGVLTDRSGRVLTFAFISNEAGPNGRNAMDALATKLWFCGCTT
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Song H, Sandie R, Wang Y, Andrade-Navarro MA and Niederweis M [2008]. Identification of outer membrane proteins of Mycobacterium tuberculosis. Localization
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
